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DESCRIPTION
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Power-Sep™
The Power-Sep™ System is a fully automated liquid chromatography
system, capable of automating the purification and production of
biomolecules using chromatography techniques such as ion exchange, gel
filtration and affinity chromatography. The Power-Sep™ System has the
unusual flexibility to run laboratory, pilot or full scale production
employing the same basic unit. The Power-Sep™ System can be expanded to
control multi-column and multi-sample applications. The DMS-6000 Data
Management Software allows real time plotting and automatic documentation
of purification data.
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FEATURES
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Chromatography Cleanup
- Real-time plotting
of UV, pH, Conductivity, and Temperature.
- Purification
parameters can be changed during the run.
- Ability to save UV,
pH, Conductivity and Temperature data every 0.5 seconds.
- Programmable flow
rate ranges 0.02ml ~ 1000ml/min.
- Saved data can be
reviewed and compressed.
- Method development
tool.
- Runs from small
scale to production scale purification.
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PRINCIPLES
OF OPERATION
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Hardware Description
The hardware consists of a computer unit and a separation unit. The
computer unit communicates with the separation unit through an RS232
interface. The separation unit consists of the following modules:
- LC Control Module.
- Pump and Pressure
Control Module.
- Valve Module.
- Valve Drive
Module.
- UV Detector.
- pH Electrode.
- Conductivity
Electrode.
- Temperature Probe.
- Gradient Module.
- Bubble Detector.
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Software
Description
The Power-Sep™ System uses Windows based DMS-6000 real time Data
Management Software to coordinate the entire purification process. The
system uses a 4 channel graphics screen for real time plotting of data,
which is obtained from a UV detector, pH and conductivity electrodes, and
a temperature probe.
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Automatic
Documentation
The Power-Sep™ can capture and save the purification data every 0.5
second in a data file. The saved plot data can be reviewed and compressed
up to 1000 times. The Power-Sep™ DMS-6000 Software allows you to display,
review and print the results of a run or a compressed summary and to
analyze trends. The report feature allows you to print the results in
graph and/or tabloid form with date, time, title, file name, and the
associated purification program.
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Graphs and Tables
The purification data; UV, pH, and conductivity, can be superimposed on
one screen. The Power-Sep™ DMS-6000 Software allows you to review saved
data from up to four runs on your display screen at once. The saved data
can be printed as a graph or a table along with the corresponding
purification program.
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Programming
The entire purification protocol from sample loading and column washing
to elution and fraction collection, can be programmed on a task by task
basis. The Power-Sep™ System can be programmed to collect fractions based
on volume, or UV absorbance. The developed protocol can be run
unattended.
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Editor
The Power-Sep™ DMS-6000 Software Windows based Editor allows you to
program multiple step purification processes in thousands of
configurations. The purification programs can be individually named and
saved. With the DMS editor you can set the following parameters:
- Flow Rate
- Volume/Time
- Input Valve
Selection
- Output Valve
Selection
- Peak Separation
- Gradient
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Wide Range
Of Flows
The Power-Sep™ System allows you to program a range of flow rates from
0.020 to 1000 ml/minutes with the same basic unit. For large scale
purifications, higher flow rate pumps are available. For details consult
FMS.
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Variety of Different
Pump Heads
The Power-Sep™'s modular pump head design allows you to choose the best
size and type of pump head for your primary process and optional pump
heads for other processes. We offer a variety of different pump head
materials including Teflon, ceramic, stainless steel, kynar and tygon.
Pump heads can be manually replaced in less than a minute.
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Works with Organic
Solvents
By replacing the pump head, and valve modules, the same Power-Sep™ system
can perform applications that require organic solvents. FMS’s wide range
of pump heads and valve materials allow you to use any kind of organic
and inorganic reagents.
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Precision
The heart of the Power-Sep™ System is FMS's patented calibration process.
It is capable of learning the optimum purification conditions and
reproducing the same purification conditions over and over again.
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UV Detector, pH,
& Conductivity Electrodes
The Power-Sep™ System monitors the UV absorption, conductivity,
temperature, and pH of the purified material during the run. The data
obtained from the detectors is plotted in a multi-channel plotting screen
and can be stored for future reference or print out.
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Columns
The columns are constructed from either PTFE, glass or acrylic. They can be
used for both bench top analytical scale and floor mounted large scale
separations. The columns are engineered in a variety of different sizes
for gel filtration, affinity or ion exchange techniques.
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Scale-up
Unlike other commercial systems, which have been marketed for either
laboratory, pilot, or process scale purification, the Power-Sep™ System
is designed to be used from laboratory to production scale purification
with the same basic unit. The modularity of the Power-Sep™ allows the
transition from small to large scale purification to be as easy as
replacing the pump and the valve modules. This feature of the Power-Sep™
System saves money and makes the process development and scale-up an
easier task.
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Linear Gradient
The low pressure linear binary and ternary gradient module allows the
creation and storage of unlimited numbers of profiles. The gradient
module is interfaced to the Power-Sep™ System and controls the gradient
valve module for gradient formation.
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Power-Sep™ System Configurations:
System #1
If you are performing your purifications manually and the high cost of
automation has prevented you from automating your purifications, you will
love our System #1 configuration. This low cost system interfaces to your
existing UV, column and chart recorder and automates your entire
purification process.
System #2
If you are involved with analytical scale purification, our System #2
configuration offers you a complete laboratory scale purification system.
System #3
The modular system design of the Power-Sep™ System allows you to
reconfigure and expand the system to suit all your purification needs.
System #4
For method development and scale-up stages, Power-Sep™ System #4 offers
you the DMS-6000 complete documentation and analysis tools package. This
software package assists you in creating an optimized protocol for your
purification process.
System #5
As your purification application needs grow from laboratory to pilot to
process scale, System #5 can be used in all stages of scale-up. With
System #5 it is possible to run columns ranging from 1 ml to hundreds of
liters.
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For additional
Information please contact FMS at onlineinfo@fms-inc.com
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